Abstract

Periodontal disease is a multifactorial condition characterized by chronic inflammation and progressive destruction of supporting tissues, with significant implications for oral health and systemic well-being. This study investigated the correlation between inflammatory responses and regenerative outcomes in periodontal supporting tissues, emphasizing the role of epigenetic regulation. A mixed methods approach was employed, combining clinical assessments, patient-reported outcomes, and molecular analyses. Spearman correlation analysis revealed strong associations between clinical indicators of inflammation and perceived regenerative improvements, underscoring the interplay between destructive and reparative processes. Demographic and lifestyle factors, including smoking and systemic health conditions, were found to influence variability in responses. Regenerative therapies demonstrated favorable outcomes in terms of clinical attachment gain, alveolar bone support, and patient satisfaction. These findings highlight the importance of integrating epigenetic insights with clinical practice to develop personalized strategies for managing periodontal disease and enhancing tissue regeneration

Abstract

Obesity and headache are conditions associated with a substantial personal and societal impact. This study aims to evaluate the association between body mass index (BMI) and Headache among adults in Gharyan City, Libya. This was a descriptive cross-sectional study, conducted at Gharyan city, Libya, during the period from December 2024 to March 2025. Two hundred (200) participants, including 94 males (47%) and 106 females (53%), were enrolled in this study. Data were collected by using a structured questionnaire. The participants were divided into four categories, based on BMI: underweight (<18.5), normal weight (18.5 to 24.9), overweight (25 to 29.9), and obese (>30). A total of 200 participants, both gender males and females, were enrolled in this study during the study period. The study indicates that high percentage of headache in age was 43 -56 years, and severe obesity BMI > 30 kg/m2 were observed of high percentage in females n=27 (25.47%) compared to males n=17 (18.08%), although the severe obesity and headache found in females n=37 (34.90%), rather than males n=30 (31.91%) Obesity and headache are both, slightly more common in females than males, we recommend that more studies among obesity and headache in large patients should be done, although small sample size and lack of long-term flow-up of patients, were the main limitations of our study and results suggested a need for monitoring by X-ray and MRI diagnosis of patients have obesity

Keywords. Obesity. Headache, Gharyan City, Libya.

Abstract

Natural dentin repair is a biologically mediated process that preserves tooth vitality through odontoblast activity and intracellular signaling pathways. This study employed a mixed methods design, combining laboratory experiments with descriptive analytical surveys of dentists in Egypt, to investigate the mechanisms of odontoblast differentiation and assess clinical knowledge and practices related to regenerative dentistry. Laboratory analyses included microscopic and molecular techniques to evaluate cellular signaling during dentin repair, while questionnaires were distributed to 100 dentists across public and private sectors. Data were analyzed using quantitative statistics, qualitative assessments, and integrative interpretation of laboratory and clinical findings. Results revealed significant positive correlations between dentists’ knowledge of intracellular signaling and their clinical practices, with Spearman correlation coefficients ranging from 0.52 to 0.65 (p = 0.01). A majority of respondents (80%) reported experience with mesenchymal stem cell therapy for craniofacial bone remodeling or dental tissue regeneration, and 70% observed improvements in bone density, while 65% reported enhanced dental tissue regeneration. Imaging studies were widely used, with 85% of participants undergoing radiographic or CT evaluations to monitor progress. Despite these encouraging outcomes, only 35% of dentists were familiar with molecular techniques targeting signaling pathways, and just 40% reported applying clinical strategies to enhance odontoblast differentiation. Notably, 75% expressed interest in specialized training to improve their knowledge and practices in regenerative dentistry. The study concludes that while theoretical awareness of dentin repair mechanisms is relatively strong, clinical application remains limited. Bridging this gap through structured training and evidence-based protocols will be essential for integrating molecular insights into routine dental practice, thereby improving dentin repair outcomes and long-term tooth vitality.

Abstract

Stem cell therapy has emerged as a promising approach for craniofacial bone remodeling and dental tissue regeneration. In this study, a mixed-methods design was employed, combining laboratory analyses with clinical data from 100 participants. Results demonstrated that 80% of respondents had received mesenchymal stem cell (MSC) therapy, with 70% reporting improvements in bone density and 65% noting enhanced dental tissue regeneration. Imaging tests confirmed progress in 85% of cases, while overall satisfaction with therapy outcomes was reported by 80–85% of participants. Correlation analysis revealed strong positive relationships between stem cell treatment and improvements in bone and dental tissues, with coefficients ranging from 0.60 to 0.70 (p < 0.01). Side effects were minimal, with only 20% reporting complications such as swelling or pain. These findings underscore the therapeutic potential of stem cell-based interventions, highlighting both their efficacy and safety. The study concludes that stem cell therapy represents a viable and effective strategy for regenerative dentistry and craniofacial surgery, though larger multicenter trials are needed to standardize protocols and validate long-term outcomes.

Abstract Antimicrobial agents are widely used to treat bacterial infections; however, their misuse contributes to the growing problem of antibiotic resistance. This study assessed the prevalence of antibiotic resistance among bacterial isolates from patients in Gharyan City, Libya, and explored behavioral factors influencing resistance. Between January and June 2024, 66 clinical samples (urine, stool, vaginal swabs, blood, pus, and sputum) were collected from seven medical laboratories. A questionnaire survey was conducted among three groups: medical workers (n=50), non-medical individuals (n=50), and pharmacists (n=50). Laboratory identification employed selective media, Gram staining, and microscopy, while antibiotic susceptibility was tested using disc diffusion. Results revealed widespread inappropriate antibiotic use: 70% of pharmacists dispensed antibiotics without prescriptions, 40% provided incorrect dosages, and only 10% held formal pharmacy qualifications. Among medical workers, 40% used antibiotics without prescriptions and 56% failed to complete treatment courses. Infections were more common in females (77%) than males (23%), with the highest prevalence in younger age groups (<32 years, 39.4%). Staphylococcus spp (32%) and E. coli (27%) were the most frequent isolates. Overall resistance was high (75%), particularly in Pseudomonas spp (100%), E. coli (83%), and Staphylococcus spp (76%). Azithromycin (67%) and Doxycycline (49%) showed the highest resistance rates, while Meropenem (2%) and Nalidixic acid (3%) remained largely effective. These findings highlight alarming levels of antibiotic resistance in Gharyan, driven by misuse and poor prescribing practices. Strengthening pharmacist and physician training, enforcing prescription regulations, and raising public awareness are essential to mitigate resistance and preserve antibiotic efficacy. Keywords. Antibiotic Resistance, Bacterial Infection, Misuse of Antibiotics, Control and Surveillance.

ABSTRACT This study investigates the effects of self-assessment on students’ motivation and language proficiency among English Department students at the Faculty of Arts, AL-Assaba. A quantitative research design was adopted, using a questionnaire distributed to a sample of 60 students. The findings indicate that self-assessment plays a significant role in enhancing learners’ motivation, self-awareness, and perceived language proficiency. Most students reported positive attitudes toward self-assessment and felt it Improved their English skills. The study concludes that incorporating self-assessment into English language instruction can foster learner autonomy and Improve learning outcomes. It recommends Integrating self-assessment practices into curricula and providing guidance to students on effective self-assessment. Keywords: Self-assessment, Motivation, Language Proficiency, Learner Autonomy, English Department Students, Higher Education.