Chamomile (Matricaria chamomilla L.) is a medical plant known for its antioxidant properties, which are attributed to bioactive compounds such as flavonoids and terpenoids. The study aimed to compare the antioxidant efficacy of natural chamomile with three commercial brands, A (Italian chamomile (Sonny)), B (Italian chamomile (Restora)), and C (German chamomile (UTZ)) available in Libyan markets. Methanol extracts of all samples were prepared by maceration, and antioxidant activity was evaluated using the DPPH radical scavenging assay. All data were expressed as mean ± standard deviation (SD). Statistical analysis was performed using one-way ANOVA, with A p-value < 0.05, which was considered statistically significant. Results revealed significant differences in IC50 values, with natural chamomile exhibiting the highest potency (IC50 = 3.35 mg/ml ± 0.055), followed by Brand A (IC50= 3.56 mg/ml ± 0.04) and Brand C (3.88 mg/ml ± 0.21). Brand B showed the weakest activity (IC50= 5.01 mg/ml ± 0.04). The statistical analysis confirmed the superiority of natural chamomile over commercial variants. Our findings suggest that processing methods, storage conditions, or potential additives in commercial products may degrade bioactive compounds, reducing antioxidant efficacy. This study highlights the advantage of minimally processed natural chamomile for optimal antioxidant benefits, and it underscores the implications for consumer choice and quality control in the manufacturing of herbal products.

Abstract: Vitamin D deficiency is a public health concern affecting many individuals as it is highly prevalent in

all parts of the world. Recent studies have reported an association of vitamin D deficiency with cardiometabolic

alterations such as dyslipidemia. The study aimed to evaluate vitamin D and lipid profile levels among the Libyan

adult population and investigate the correlation of vitamin D deficiency with the alteration of lipid profile levels.

A cross-sectional study was conducted at Janzour Hospital among 193 subjects (107 males and 86 females) whose

ages ranged between 20 and 50 years over three months from Sept to Dec 2023. Vitamin D, cholesterol,

triglyceride, low-density lipoprotein, and high-density lipoprotein levels were estimated. The total mean vitamin

D levels were 8.31±4.74, 23.83±2.78, and 42.67±7.95 ng/dl for deficiency, insufficiency, and sufficiency,

respectively. The findings revealed significant alterations in cholesterol (increase), triglyceride (increase), LDLcholesterol

(increase), and HDL-cholesterol (decrease) among subjects who had vitamin D deficiency or

insufficiency as compared to subjects having vitamin D sufficiency. Vitamin D levels were negatively correlated

with cholesterol, triglyceride, and LDL cholesterol, and they were positively associated with HDL cholesterol.

The incidence of dyslipidemia is higher in the vitamin D deficiency group than in the insufficiency and sufficiency

groups. It is essential to frequently monitor lipid profiles among vitamin D-deficient individuals to avoid

subsequent disorders or damages associated with the alterations of lipid profile patterns.

Andrographolide (AG) has been shown to have several medicinal and pharmaceutical effects, such as antimicrobial, anti-inflammatory, antioxidant, anti-diabetic, and anti-malarial activities. Moreover, studies to assess the pharmacological effect of AG on the metabolic changes of uninfected red blood cells (uRBCs) have not yet been investigated. This study aims to evaluate the pharmacological effects of AG compared to chloroquine (CQ) on the metabolic variations of uRBCs in vitro using a proton nuclear magnetic resonance (1H-NMR)-based metabolomics approach coupled with multivariate data analysis (MVDA). Forty-one metabolites were successfully identified by 1H-NMR. The results of the unsupervised data analysis principal component analysis (PCA) showed ideal differentiation between AG and CQ. PC1 and PC2 accounted for 71.4% and 17.7% of the explained variation, respectively, with a total variance of 89.10%. Based on S-plot and VIP values, a total of 28 and 32 metabolites were identified as biomarkers in uRBCs-AG and uRBCs-CQ, respectively. In uRBCs treated with AG, ten metabolic pathways were determined to be disturbed, including riboflavin metabolism, d-glutamate and d-glutamine metabolism, phenylalanine metabolism, glutathione metabolism, proline and arginine metabolism, arginine biosynthesis, citrate cycle, glycolysis/gluconeogenesis, and pyruvate metabolism as well as alanine, aspartate, and glutamate metabolism. In contrast, in CQ-treated uRBCs, nine affected metabolic pathways were determined, which involved the same metabolic pathways for uRBCs-AG, except for glutathione metabolism. These findings suggest an evident relationship between AG and CQ associated with metabolic changes in intact RBCs after being exposed to the treatment. The metabolomics results could allow useful comprehensive insights into the underlying mechanism of the action of AG and CQ on red blood cells. Consequently, the 1H-NMR-based metabolomics approach was successfully utilized to identify the pharmacological effects of AG and CQ on the metabolic variations of uRBCs.