• Abstract

Corticotropin-releasing factor (CRF) orchestrates our body's response to stressful stimuli. Pain is often stressful and counterbalanced by activation of CRF receptors along the nociceptive pathway, although the involvement of the CRF receptor subtypes 1 and/or 2 (CRF-R1 and CRF-R2, respectively) in CRF-induced analgesia remains controversial. Thus, the aim of the present study was to examine CRF-R1 and CRF-R2 expression within the spinal cord of rats with Freund's complete adjuvant-induced unilateral inflammation of the hind paw using reverse transcriptase polymerase chain reaction, Western blot, radioligand binding, and immunofluorescence confocal analysis. Moreover, the antinociceptive effects of intrathecal (i.t.) CRF were measured by paw pressure algesiometer and their possible antagonism by selective antagonists for CRF-R1 and/or CRF-R2 as well as for opioid receptors. Our results demonstrated a preference for the expression of CRF-R2 over CRF-R1 mRNA, protein, binding sites and immunoreactivity in the dorsal horn of the rat spinal cord. Consistently, CRF as well as CRF-R2 agonists elicited potent dose-dependent antinociceptive effects which were antagonized by the i.t. CRF-R2 selective antagonist K41498, but not by the CRF-R1 selective antagonist NBI35965. In addition, i.t. applied opioid antagonist naloxone dose-dependently abolished the i.t. CRF- as well as CRF-R2 agonist-elicited inhibition of somatic pain. Importantly, double immunofluorescence confocal microscopy of the spinal dorsal horn showed CRF-R2 on enkephalin (ENK)-containing inhibitory interneurons in close opposition of incoming mu-opioid receptor-immunoreactive nociceptive neurons. CRF-R2 was, however, not seen on pre- or on postsynaptic sensory neurons of the spinal cord. Taken together, these findings suggest that i.t. CRF or CRF-R2 agonists inhibit somatic inflammatory pain predominantly through CRF-R2 receptors located on spinal enkephalinergic inhibitory interneurons which finally results in endogenous opioid-mediated pain inhibition.

Abstract

The rock units of Wadi El-shati were classified to group of clusters to explain the concentration of iron ore by using enhanced satellite image. The several images of single band of Thematic Mapper (TM7) of Landsat were combined to produce multispectral image bands. It was processed by group of filters after the false color image has been made. The filtered image was classified with unsupervised classification after the image ratio has been done by dividing band 3 on band1. Finally, we reached the classified image has concentration of iron tone.

Single carrier with frequency domain equalization (SC/FDE) substantially out performs OFDM in BER performance for Ultra Wideband (UWB), a result already reported. In this paper we provide an explanation and justify this superiority. We showed besides, by only a proper selection of both cyclic prefix duration and the period of fast Fourier transform an extra gain is obtainable. This performance enhancement is in favor of SC/FDE's superiority for UWB in the context of internet of things.

• Experimental and theoretical comparisons have been performed for natural convection heat transfer over rectangular fins array at different fin parameters. This investigation includes the effect of fin length, fin spacing, fin height, orientation angle, and temperature difference between the heat sink and the surrounding environment. To understand the general flow patterns dominating flows from the heat sink, the three dimensionless elliptic governing equations were solved using finite volume computational fluid dynamics (CFD) code, and the experimental work was carried for the system at different orientations. A new empirical correlation (modified of McAdam's correlation) was derived to correlate the mean Nusselt number as a function of the Rayleigh number. The average heat transfer coefficient has a maximum value at an orientation angle equal to zero degrees, and it decreases with an increasing orientation angle. The heat transfer rate per unit base area increases as fin spacing increase until it reaches a maximum value (6.5 mm), then it decreases with a further increase of fin spacing. The results of these investigations between the experimental and theoretical study were showing good agreements with similar international works.