Heavy metals can be released from cooking stewpots into food, causing food contamination and posing a risk to human health. This research study investigated the release of heavy metals into food from stewpot cookers used in Gharyan, Libya. As well as, investigating the effect of scratches, acidic food, and storage on the concentrations of those metals. Eight new and used cooking stewpots (with scratches) made of: (Tefal, stainless steel, copper and aluminum) were collected, and then fresh tomato juice was cooked in these stewpots and left in the refrigerator for six days, the samples were digested and analyzed by atomic absorption spectrophotometer (AAS) to measure the concentration of the heavy metals: (cadmium, lead, iron, copper and zinc). Results showed that cadmium and lead concentrations were less than the detection limit of the AAS instrument, except for one sample (old Tefal stewpot with scratches), in which the lead concentration was (0.046ppm). The concentration of iron, copper and zinc in the samples was less than the maximum limit allowed by WHO/FAO; except for one sample in which the iron concentration exceeded that value (old copper pot with scratches) and its value was (2.892ppm). The results of the descriptive statistical analysis showed that the average concentration of iron (0.493 ppm) was higher than that of zinc and copper (0.054 ppm), (0.016 ppm), respectively. In addition, the rate of leakage of those elements from old (scratched) cookware was higher than that from the new ones made from the same material, and the concentration of these elements increases significantly when these stewpots contain scratches, since that increases the rate of leakage of those metals, especially in acidic food such as tomatoes.

Thyroid function is impaired by diabetes, resulting into thyroid disorders, accompanied by the alteration of thyroid-stimulating hormone (TSH) and thyroxine (T4) levels. The aim of this study was to investigate the role of vitamin D supplementation on TSH and total T4 levels in serum of Libyan patients suffering from type 2 diabetes mellitus (T2DM) with thyroid disorders. A total of 180 subjects were recruited and distributed into two groups: 80 participants in control group (43 females and 37 males), with an age range of 26-72 years, and 100 participants in T2DM patient group (54 females and 46 males), with an age range of 26-63 years. Each T2DM patient received a monthly intramuscular injection of 200000 ng/ml vitamin D for three months. Glucose, vitamin D, total T4 and TSH levels were determined in serum samples of healthy subjects and of T2DM patients (pre- and post-administration of vitamin D). After the T2DM group received vitamin D supplementation, the mean of vitamin D level increased in the T2DM, while the mean of glucose level decreased. Most importantly, the mean of total T4 level for patients increased significantly (p ˂ 0.05) from 1.34 to 8.23 g/dL, both of which were lower than the control group mean 10.99 g/dL. In contrast, the mean of TSH level for patients decreased significantly (p ˂ 0.05) from 11.77 to 2.71 mU/L, and both values were greater than the control group mean 2.01 mU/L. ANOVA results showed that age, gender, and body mass index had no significant individual interactions (p > 0.05) with vitamin D supplementation on total T4 and TSH levels. Vitamin D supplementation had a beneficial role on total T4 and TSH levels in serum of Libyan T2DM patients with thyroid disorders.