ABSTRACT

INTRODUCTION: Malignancy is regarded as a potentially fatal disorder that can impact human health globally. More than 90% of all oral cavity malignancies that have been detected are invasive oral squamous cell carcinomas (OSCCs). Patients having OSCC still have an extremely poor survival rate, even though medical therapy has advanced significantly. Medicinal plants have been the subject of recent scientific research. Capparis spinosa L. (CS) is among the prominent plants found across the Mediterranean region.According to phytochemical analysis, CS leaves are considered to be a promising therapeutic plant since they are an essential spring of bioactive substances such as flavonoids and phenolic acids. Research has indicated that CS extract exhibits anti-carcinogenic properties against numerous forms of cancer.

OBJECTIVE: The current research was to the evaluation of the anti-carcinogenic effect of the ethanolic extract of CS leaves on SCC-4 cell lines contrasted with both the untreated cancer cell lines and Cisplatin treated.

MATERIALS AND METHODS: This work comprised an in vitro investigation. SCC-4 cell line was separated into 3 groups: group 1 was considered as negative control (untreated), group 2 was positive control (Cisplatin-treated), and group 3 (treated with CS leaves extract). Viability, proliferation and apoptosis assays were performed to evaluate the possible therapeutic effect of the CS leaves extract in duration of 48 hours.

RESULTS: Treating SCC-4 cell line with the extract of CS leaves has shown that the viability effect was in a dose-dependent fashion. The IC50 or the half-maximal inhibitory concentration was equal to 176.3μg/ml. Also, CS treatment has elevated the apoptotic cells’ percentage and decreased SCC-4 cells proliferation rate in compare to the untreated cancer cell lines and Cisplatin

action.

CONCLUSION: the extract of CS leaves may have a prominent anti-cancerous effect on OSCC.

KEYWORDS: Oral squamous cell carcinoma, Capparis spinosa L., Viability, Proliferation, Apoptosis.

Running Title: Anti-carcinogenic effect of Capparis spinosa L. on SCC-4

Abstract

Objectives: The goal of this study was to determine the correlation of clinicopathological factors and the up-regulation of Int-8 receptors and vascular endothelial growth factor (VEGF) expression in oral squamous cell carcinoma (OSCC).

Materials and Methods: Clinical data (age, gender, size of the tumor, location of the tumor, lymph nodes status,..) were collected from 20 patients with OSCC, tabulated and statistically analyzed (SPSS17).Immunohistochemical stainings of Int-8 receptors, CXCR1 and CXCR2 (united states biological, United Bio, USA) and VEGF (Abcam, Cambridge, MA, USA) were done.

Results: Our immunohistochemical study demonstrated: 1)expression of Int-8 receptors in all cases of OSCC with variable intensities, 2) high-level staining of VEGF In poorly differentiated and invasive oral squamous cell carcinoma. Significant correlation was observed between immunohistochemical expression of Int-8 receptors, VEGF and histologic differentiation and clinical stages(Analysis of variance ANOVA, P<0.05).

Conclusion: Our findings suggest that the reactivity to Int-8 receptors

observed in OSCC cases could be used as a parameter for tumor aggressiveness and as potential prognostic marker. Also, up-regulation of VEGF may play a role in the angiogenesis and progression of oral squamous cell carcinoma.

Background:. Interleukin-8 (IL-8) is a pro-inammatory cytokine which exerts its effects via binding to CXCR1 and

is known to promote angiogenesis. CXCR1 represent potential prognostic biomarkers and therapeutic target.

Aim of the study: To evaluate the expression of CXCR1 in OSCC and control tissue as well as correlate the expression of the CXCR1to the

clinicopathologic parameters of OSCC .

Material and Methods: Immunohistochemical analysis of 22 cases OSCC and 7 control cases sections stained by anti-CXCR1antibody.

Immunohistochemical staining will be performed using a Labeled Strept-Avidin Biotin complex method (LSAB).

Results: Expression was recognized in all cases of OSCC and normal control cases and the pattern of expressed in both the cytoplasm and

nucleus of the malignant epithelial cells (total cell reactivity), as well as high expression was noticed in low histological grade and clinical

stage malignancy, whereas decrease of expression was detected in high grade and stage malignancy.

Conclusions: the results show an association between CXCR1and the clinicopathologic parameters, and considered as prognostic marker

in OSCC